fluorescence resonance energy transfer (fret) microscopy data storage (Neurodata GmbH)
Structured Review
. ( d ) Neurodata Without Borders (NWB) provides a robust, extensible, and maintainable software ecosystem for standardized description, storage, and sharing of the diversity of experimental subjects, behaviors, experimental designs, data acquisition systems, and measures of neural activity exemplified in a – c .](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1949/pmc09531949/pmc09531949__elife-78362-fig1.jpg)
Fluorescence Resonance Energy Transfer (Fret) Microscopy Data Storage, supplied by Neurodata GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: The Neurodata Without Borders ecosystem for neurophysiological data science
Journal: eLife
doi: 10.7554/eLife.78362
Figure Legend Snippet: ( a ) Diversity of experimental systems: species and tasks: (i) mice performing a visual discrimination task; (ii) rats performing a memory-guided navigation task; (iii) humans speaking consonant-vowel syllables; (iv) biophysically detailed simulations of mouse hippocampus during memory formation. The corresponding acquisition modalities and signals are shown in the corresponding columns in figure ( b and c ). ( b ) Diversity of data modalities and acquisition devices: (i) optophysiological Ca 2+ imaging with two-photon microscope; (ii) intra-cortical extracellular electrophysiological recordings with polytrodes in multiple brain areas (indicated by color, see c.ii); (iii) cortical surface electrophysiology recordings with electrocorticography grids; (iv) high-performance computing systems for large-scale, biophysically detailed simulations of large neural networks. ( c ) Diversity of signals and areas: (i) Ca 2+ signals as a function of time from visually identified individual neurons in primary visual cortex (V1) ; (ii) spike-raster (each tick demarcates the time of an action potential) from simultaneously recorded putative single-units after spike-sorting of extracellular signals from medial prefrontal cortex (mPFC; blue), ventral striatum (v. Striatum, red), and orbital frontal cortex (OFC, green) (color corresponds to b.ii ) ; (iii) high-gamma band activity from electrodes over the speech sensorimotor cortex (SMC), with dorsal-ventral distance from Sylvian fissure color coded red-to-black (color corresponds to b.iii) ; (iv) simulated intracellular membrane potentials from different cell-types from large-scale biophysical simulation of the hippocampus (BC, Basket Cell); HC, Hilar Interneuron (with axon associated with the) Perforant Path; HCC, Hilar Interneuron (with axon associated with the) Commissural/Associational Path; IS, Interneuron-Specific Interneuron; MCPP, medial Perforant Path; NGFC, neurogliaform cell; MC, mossy cell; GC, granule cell](Raikov and Soltesz, unpublished data). ( d ) Neurodata Without Borders (NWB) provides a robust, extensible, and maintainable software ecosystem for standardized description, storage, and sharing of the diversity of experimental subjects, behaviors, experimental designs, data acquisition systems, and measures of neural activity exemplified in a – c .
Techniques Used: Imaging, Microscopy, Activity Assay, Membrane, Software